Ca2+ oscillatory pattern in fertilized mouse eggs affects gene expression and development to term
نویسندگان
چکیده
منابع مشابه
Full-term development after transplantation of parthenogenetic embryonic nuclei into fertilized mouse eggs.
Diploid parthenogenetically activated oocytes were obtained after gonadotropin-induced ovulation of virgin females of the LT/Sv (LT) inbred mouse strain. These oocytes cleave spontaneously and develop into blastocysts which implant in the uterus but die within a few days. We examined the developmental potential of nuclei from parthenogenetic embryos after transplantation into fertilized eggs. T...
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Fi(CBA x C57BL/10) mouse eggs originating from spontaneous or induced ovulation and fertilized by CBA-T6T6 or PO spermatozoa were bisected with a glass needle into halves each containing a pronucleus.This technique offers a unique opportunity of producing both androgenetic and gynogenetic haploid embryos from one egg. Out of 600 operated eggs, in 406 (67-7%) both halves survived. During 96 h of...
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When fertilized one-cell eggs are subjected to distilled water treatment for 2-6 min, cytoplasm bulges through the sperm-slit in the zona pellucida and forms a cytoplasmic fragment (CF). CFs were observed in 86-5% of eggs; in 20-9% of cases CFs contained a pronucleus (or pronuclei). In 53-4% of eggs permanent incorporation of the second polar body (2 P.B.) into the egg cytoplasm occurred. These...
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ژورنال
عنوان ژورنال: Developmental Biology
سال: 2006
ISSN: 0012-1606
DOI: 10.1016/j.ydbio.2006.08.041